Current Issue

A simple, precise, accurate, and robust Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative determination of Tedizolid in pharmaceutical dosage forms. Method development trials were performed using different mobile phase compositions of ethanol and water on a C?? (250 × 4.6 mm, 5 µm) column. Among the tested conditions, the optimized chromatographic system consisted of DMSO: Buffer (90:10 v/v) in isocratic mode at a flow rate of 1.0 mL/min, with detection at 296 nm and an injection volume of 20 µL. The retention time of Tedizolid was found to be approximately 2.97 minutes.The method was validated as per ICH Q2 guidelines. System suitability parameters were within acceptable limits, with plate count around 3000 and tailing factor approximately 1.25. The method exhibited good linearity in the concentration range of 10–60 µg/mL with a correlation coefficient (r) of 0.9966. Precision studies showed %RSD less than 2% for system precision, method precision, and intermediate precision. Accuracy was confirmed by recovery studies at 50%, 100%, and 150% levels, yielding recoveries between 99.31% and 100.18%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 22.85 µg/mL and 69.30 µg/mL, respectively. Solution stability studies indicated that the analyte remained stable for up to 16 hours.The assay of the marketed formulation (StariZo) showed 99.9% purity. The developed method was found to be specific, sensitive, reproducible, and suitable for routine quality control analysis of Tedizolid in bulk and pharmaceutical formulations.

Objective: The objective of the present investigation was to formulate and evaluate a polyherbal topical cream containing extracts of Lactuca sativa and Curcuma longa with analgesic and antimicrobial potential. Methods: An oil-in-water topical cream was formulated using beeswax and borax as emulsifying agents. Polyherbal extracts were incorporated into the cream base and five formulations (F1–F5) were prepared by varying excipient concentrations. The formulations were evaluated for physicochemical parameters such as pH, spreadability, viscosity, homogeneity, washability, acid value, saponification value, and phase separation. In-vitro drug release was studied using a Franz diffusion cell. Results: Among the prepared 5 batches of herbal formulation, batch 4 exhibited optimal physicochemical characteristics, possessing pH 5.8, spreadability of 20gcm/sec, and a viscosity of 2500 cps, which lies within an acceptable limits with no phase separation, compared to other batches. Conclusion: The formulated polyherbal cream demonstrated acceptable pharmaceutical properties and potential therapeutic utility. In vitro study for drug release was showed good acceptance of optimized formulation. Further pharmacological and antimicrobial studies are warranted.

Ziziphus mauritiana Lam. (Badar), commonly known as Ber or Indian Jujube, is an important plant in Ayurveda. Ancient texts mention it for gastrointestinal, respiratory, gynecological, and skin conditions.[2-9] Its rasapanchaka describes madhura rasa, sheeta veerya, madhura vipaka and vatapittashamaka action.[2-4] Classical references include Atisara (diarrhea), Pravahika (bloody diarrhea), Kasa (cough), Shwasa (asthma), Jwara (fever), Raktapradara (menorrhagia), Arati(fatigue), Splenomegaly, and Masurika(pox).[2-8] Modern pharmacology supports these claims, highlighting antioxidant, antimicrobial, antidiabetic, analgesic, anti-inflammatory, sedative, hepatoprotective, and cytotoxic activities.[11-16]

The present study focuses on the formulation and evaluation of a herbal hair serum and its preliminary phytochemical, physicochemical, spectral, antimicrobial, and hair regrowth activities. Preliminary phytochemical screening revealed the presence of alkaloids, carbohydrates, tannins, flavonoids, glycosides, saponins, and phytosterols, while proteins, terpenoids, and phenols were absent. Evaluation parameters indicated that the serum was dark brown in color, aromatic, smooth in texture, and had a pH of 8.2 with no irritation potential. The specific gravity (1.096), viscosity (0.91 cps), saponification value (291.72), and spreadability (9 cm) suggested suitable formulation characteristics. UV–Visible spectrophotometric analysis showed maximum absorbance (0.870) at 296 nm, identified as ?max, with negligible interference in the visible region. IR spectral analysis confirmed the presence of functional groups corresponding to bioactive compounds. The formulation exhibited significant antifungal activity against Trichophyton spp. (10 mm) and Candida albicans (12 mm), and antibacterial activity against Staphylococcus aureus (10 mm) and Escherichia coli (22 mm). Hair regrowth studies demonstrated comparative effectiveness with standard treatment, indicating the potential of the herbal hair serum as a natural therapeutic alternative.

Lasmiditan is a novel, centrally acting selective 5-HT?F receptor agonist approved for the acute treatment of migraine with or without aura. Unlike triptans, it does not cause vasoconstriction, making it a safer therapeutic option for patients with cardiovascular risk factors. Chemically, lasmiditan is 2,4,6-trifluoro-N-[6-(1-methylpiperidine-4-carbonyl)-pyridin-2-yl] benzamide with a molecular formula of C??H??F?N?O? and molecular weight of 377.367 g/mol. It is available as lasmiditan hemi-succinate in 50 mg film-coated tablets. Lasmiditan exerts its antimigraine effect by selectively activating central 5-HT?F receptors located on trigeminal neurons, thereby inhibiting calcitonin gene-related peptide (CGRP) release and suppressing nociceptive transmission within the trigeminovascular pathway. The drug demonstrates rapid oral absorption, moderate protein binding, central nervous system penetration, hepatic non-CYP metabolism, and an elimination half-life of approximately 5–7 hours. Common adverse effects include dizziness, fatigue, somnolence, and paresthesia. Various analytical methods such as UV–Visible spectroscopy, HPLC, RP-HPLC, and LC-MS/MS have been developed and validated for its quantitative estimation, demonstrating high linearity, accuracy, and precision. This review highlights the physicochemical properties, pharmacodynamics, pharmacokinetics, mechanism of action, therapeutic uses, adverse effects, and analytical methods of lasmiditan.